Phosphorylation of tropomyosin during development in mammalian striated muscle

FEBS Letters

Moir, A.J.G.¹  Heeley, D.A.¹  Perry, S.V.¹ 

[1] Department of Biochemistry, University of Birmingham, P.O. Box 363, Birmingham B15 2TT, England

关键词: Mammalian muscle;    Tropomyosin;    Isoelectric focusing;    Phosphorylation;    Development;    Tissue culture;   

DOI  :  10.1016/0014-5793(82)80716-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

α-Tropomyosin from rat cardiac muscle was shown by two-dimensional gel electrophoresis to become phosphorylated when tissue slices were incubated in Eagle's medium supplemented with ³²P_i. In the adult rat and mouse heart the level of phosphorylation was ∼30%, but the level was much higher in the foetal heart (60–70%). A similar developmental trend was observed in skeletal muscle from the rat and mouse, where phosphorylated forms of both α- and β-tropomyosins were observed. When rat cardiac cells were grown in tissue culture in the presence of ³²P_i, radioactivity was incorporated into the region of the gel containing tropomyosin.

【 授权许可】

Unknown