Final Technical Report

Browning, Karen S.

University of Texas

关键词: In Vitro;    59 Basic Biological Sciences;    In Vivo;    Proteins;    Phosphorylation;   

DOI  :  10.2172/841242
RP-ID  :  DOE/ER/20283F
RP-ID  :  FG03-97ER20283
RP-ID  :  841242

美国|英语

来源: UNT Digital Library

【 摘 要 】

The long term goal of this laboratory is to elucidate a detailed molecular description of the process of initiation of protein synthesis and its regulation. The specific goals of the project were: (1) development of an in vivo [{sup 32}P]- and/or [{sup 35}S]-labeling system for proteins using Arabidopsis suspension cells; (2) develop an in vitro protein synthesis assay from Arabidopsis suspension cells; (3) develop an assay for locating Arabidopsis kinases that phosphorylate the initiation factors; and (4) begin to identify Arabidopsis kinases that are involved in phosphorylation of the initiation factors.