Isolation and purification of wheat germ agglutinin and analysis of its properties

1st International Global on Renewable Energy and Development

Wang, Han^1

School of Life Science, Shandong University, SHANDONG

250013, China^1

关键词: High-efficiency;    Iso-electric points;    Isoelectric focusing;    Isolation and purification;    Physicochemical property;    Relative molecular mass;    SDS-polyacrylamide gel electrophoresis;    Wheat germ agglutinin;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/100/1/012021/pdf
DOI  :  10.1088/1755-1315/100/1/012021

来源: IOP

【 摘 要 】

In this paper, the wheat germ agglutinin was isolated and purified by affinity chromatography of chicken ovomucoid as ligand. The physicochemical properties were analyzed. The chicken ovomucoid was isolated from egg white and conjugated to affinity chromatography column agarose gel to prepare affinity adsorbent. The crude extract of wheat germ was freezedried by affinity chromatography. The physicochemical properties were analyzed by SDSpolyacrylamide gel electrophoresis and isoelectric focusing electrophoresis. And the relative molecular mass and isoelectric point of wheat germ agglutinin were obtained, and the high efficiency of purification of wheat germ agglutinin was proved by affinity chromatography.