Phosphorylation of smooth muscle myosin light chain by five different kinases

FEBS Letters

Huang, Kuo-Ping¹  Singh, Toolsee J.¹  Akatsuka, Akira¹ 

[1] National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20205, USA

关键词: Phosphorylation;    Myosin light chain;    Calcium;    Cyclic AMP;    Isoelectric focusing;    Casein kinase;   

DOI  :  10.1016/0014-5793(83)80449-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phosphorylation of the 20 kDa myosin light chain from smooth muscle by five different kinases was investigated. Three of the kinases (myosin light chain kinase, phosphorylase kinase, and cAMP-dependent protein kinase) phosphorylate serine residues, the fourth (casein-kinase-2) mainly threonine, and the fifth (glycogen synthase (casein) kinase-1) both serine and threonine. Isoelectric focusing analyses of ³²P-labelled chymotryptic peptides indicate that phosphorylase kinase and cAMP-dependent protein kinase phosphorylate the same site as myosin light chain kinase. However, both casein kinase-2 and glycogen synthase (casein) kinase-1 phosphorylate different sites.

【 授权许可】

Unknown