Alkaline phosphatase in nasal secretion of cattle: biochemical and molecular characterisation

BMC Veterinary Research

P David Eckersall¹  Nicholas N Jonsson¹  Paul Montague³  Mark McLaughlin²  HH Caline Koh-Tan¹  M Faizal Ghazali¹ 

[1] Institute of Biodiversity, Animal Health and Comparative Medicine, University of Glasgow, Bearsden Rd, Glasgow G61 1QH, UK;School of Veterinary Medicine, University of Glasgow, Bearsden Rd, Glasgow G61 1QH, UK;Institute of Infection, Immunity and Inflammation, University of Glasgow, Bearsden Rd, Glasgow G61 1QH, UK

关键词: RT-PCR;    Isoelectric focusing;    Alkaline phosphatase;    Nasal secretion;    Bovine;   

Others  :  1119026
DOI  :  10.1186/s12917-014-0204-9

 received in 2014-05-13, accepted in 2014-08-27,  发布年份 2014

【 摘 要 】

Background

Nasal secretion (NS) was investigated as a source of information regarding the mucosal and systemic immune status of cattle challenged by respiratory disease. A method for the collection of substantial volumes (~12 ml) of NS from cattle was developed to establish a reference range of analytes that are present in the NS of healthy cattle. Biochemical profiles of NS from a group of 38 healthy Holstein-Friesian cows revealed high alkaline phosphatase (AP) activity of up to 2392 IU/L. The character and source of the high activity of AP in bovine NS was investigated.

Results

Histochemical analysis confirmed the localization of the AP enzyme activity to epithelial cells and serous glands of the nasal respiratory mucosa. Analysis of mRNA levels from nasal mucosa by end point RT-PCR and PCR product sequencing confirmed that the AP was locally produced and is identical at the nucleotide level to the non-specific AP splice variant found in bovine liver, bone and kidney. Analysis by isoelectric focussing confirmed that AP was produced locally at a high level in nasal epithelium demonstrating that AP from nasal secretion and nasal mucosa had similar pI bands, though differing from those of the liver, kidney, bone and intestine, suggesting different post-translational modification (PTM) of AP in these tissues.

Conclusions

A nasal isozyme of AP has been identified that is present at a high activity in NS, resulting from local production and showing distinctive PTM and may be active in NS as an anti-endotoxin mediator.

【 授权许可】

   
2014 Ghazali et al.; licensee BioMed Central Ltd.

【 图 表 】

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