Isolation of RNA From Peripheral Blood Cells: A Validation Study for Molecular Diagnostics by Microarray and Kinetic RT-PCR Assays � Application in Aerospace Medicine

Vu NT, Zhu H, Owuor ED, Huggins ME, White VL, Chaturvedi AK, Canfield DV, Whinnery JE

FAA Office of Aerospace Medicine - Civil Aerospace Medical Institute

关键词: RNA Isolation;    Leukocytes;    Human Whole Blood;    RT-PCR;    cDNA Microarray;    Gel Electrophoresis;    b-Actin;    Cyclophilin;    Gene Expression;    PAXgeneTM Blood RNA;   

RP-ID  :  DOT/FAA/AM-04/1

美国|英语

来源: Federal Aviation Administration

【 摘 要 】

Gene expression studies in clinical diagnostic settings involve a large number of samples collected at different time points requiring effective methods for collection, transportation, storage, and isolation of RNA to maintain the integrity of expression profiles.