Comparison on Bactericidal and Cytotoxic Effect of Silver Nanoparticles Synthesized by Different Methods

International Conference on Materials, Alloys and Experimental Mechanics 2017

材料科学;金属学;机械制造

Mala, R.^1 ; Ruby Celsia, A.S.^1 ; Malathi Devi, S.^2 ; Geerthika, S.^1

Department of Biotechnology, India^1

Department of Chemistry, MepcoSchlenk Engineering College, Sivakasi, Tamil Nadu

626005, India^2

关键词: Anti-bacterial activity;    Anti-oxidant activities;    Anticancer activities;    Bioactive compounds;    Hexadecanoic acid;    Lipid peroxidation;    Lung cancer cells;    Tamarindusindica;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/225/1/012155/pdf
DOI  :  10.1088/1757-899X/225/1/012155

学科分类：材料科学（综合）

来源: IOP

【 摘 要 】

Biologically synthesized silver nanoparticle are biocompatible for medical applications. The present work is aimed to synthesize silver nanoparticle using the fruit pulp of Tamarindusindicaand to evaluate its antibacterial and anticancer activity against lung cancercell lines. Antibacterial activity was assessed by well diffusion method. Cytotoxicity was evaluated using MTT assay. GC-MS of fruit pulp extract showed the presence of levoglucosenone, n-hexadecanoic acid, 9,12-octadecadienoic acid etc. Antioxidant activity of the fruit pulp was determined by DPPH assay, hydrogen peroxide scavenging assay and lipid peroxidation. The size of biologically synthesized silver nanoparticle varied from 50 nm to 76 nm. It was 59 nm to 98 nm for chemically synthesized silver nanoparticle. Biologically synthesized silver nanoparticle showed 26 mm inhibition zone against E.coli and chemically synthesized silver nanoparticle showed 20 mm. Antioxidant activity of fruit extract by DPPH showed 84 % reduction. The IC 50 of biologically synthesized silver nanoparticle against lung cancer cell lines was 48 µg/ml. It was 95 µg/ml for chemically synthesized silver nanoparticle. The increased activity of biologically synthesized silver nanoparticle was due to its smaller size, stability and the bioactive compounds capping the silver nanoparticle extracted from the fruit extract.