Bioactivity of Acetone Extract from Spirulina Platensis

2nd Annual Applied Science and Engineering Conference

工业技术;自然科学

Djalil, A.D.^1 ; Nurmareta, Y.^1 ; Pribadi, U.^1 ; Rahayu, W.S.^1 ; Utami, P.I.^1 ; Suwandri^2

Faculty of Pharmacy, Universitas Muhammadiyah Purwokerto, Purwokerto, Indonesia^1

Department of Chemistry, Universitas Jenderal Soedirman, Purwokerto, Indonesia^2

关键词: 3-(4 ,5-Dimethylthiazol-2-yl)-2 ,5-diphenyl tetrazolium bromide;    Acetone extracts;    Anti-oxidant activities;    Anticancer activities;    Human breast cancer;    Natural products;    Radical scavenging activity;    Spirulina platensis;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/288/1/012002/pdf
DOI  :  10.1088/1757-899X/288/1/012002

来源: IOP

【 摘 要 】

Spirulina platensis is one of natural product from alkaline water which potential as nutraceutical food. The study was performed to investigate the level of antioxidative and anticancer activity of acetone extracts from Spirulina platensis. The microalga was extracted with acetone in an ultrasonic bath. Antioxidant activity was determined by using antioxidant model, 1,1-diphenyl-2picryl hidrazyl (DPPH) radical scavenging activity. The anticancer activities were evaluated against human breast cancer (T47D) and murine leukaemia (P388) cell lines. Moreover, the 3(4,5- dimethylthiazol- 2- yl) - 2,5- diphenyl tetrazolium bromide (MTT) assay was employed to evaluate cell viability. The results provide information that the extract has low antioxidant activity (IC503345 μg/mL). The crude acetone extract showed moderate anticancer activity both in T47D and P388 cell lines, IC50379.5 and 64.3 μg/mL, respectively.