Anticancer activities of ethanol extract from the Antarctic freshwater microalga, Botryidiopsidaceae sp.

BMC Complementary and Alternative Medicine

Research Article

Sun-Mi Kim¹  Ju-Mi Hong¹  Ui Joung Youn¹  Sung-Suk Suh¹  Jung Eun Kim²  Se Jong Han³  Sung Gu Lee³  Il-Chan Kim⁴  Sanghee Kim⁴ 

[1] Division of Polar Life Sciences, Korea Polar Research Institute, 21990, Incheon, Republic of Korea;Division of Polar Life Sciences, Korea Polar Research Institute, 21990, Incheon, Republic of Korea;Department of Pharmacy, Graduate School, Sungkyunkwan University, 16419, Suwan, Republic of Korea;Division of Polar Life Sciences, Korea Polar Research Institute, 21990, Incheon, Republic of Korea;Department of Polar Sciences, University of Science and Technology, 21990, Incheon, Republic of Korea;Division of Polar Life Sciences, Korea Polar Research Institute, 21990, Incheon, Republic of Korea;Department of Polar Sciences, University of Science and Technology, 21990, Incheon, Republic of Korea;Department of Polar Ocean Environment, Korea Polar Research Institute, 21990, Incheon, Republic of Korea;

关键词: Antarctic freshwater microalga;    Botryidiopsidaceae;    Anticancer activities;    Anti-proliferation;   

DOI  :  10.1186/s12906-017-1991-x

 received in 2017-06-23, accepted in 2017-10-30,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundCancer is a leading cause of human death around the world and occurs through the highly complex coordination of multiple cellular pathways. Recent studies have revealed that microalgal extracts exhibit considerable pharmaceutical activities, including those against various cancer cells. Thus, microalgae are promising candidates as novel cancer therapeutic drugs. In this study, we evaluated the biological functions of the ethanolic extract of the Antarctic freshwater microalga, Botryidiopsidaceae sp., such as its antioxidant, anti-proliferative, apoptotic and anti-invasive properties.MethodsTo estimate antioxidant capacity of ethanol extract of Botryidiopsidaceae sp. (ETBO), free radical 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays were used. The anti-proliferative activity of ETBO was assessed in several cancer cell lines (A375, Hs578T and HeLa) and non-tumorigenic keratinocyte cells (HaCaT), using MTT assay. In addition, Annexin V binding was performed to detect ETBO-induced apoptotic cells, and the expression levels of apoptosis-regulating proteins, caspase-3, p53, and Bcl-2, were determined by western blot. Boyden chamber assays were used to determine anti-migratory and anti-invasive properties of ETBO.ResultsETBO exhibited antioxidant activity and concentration-dependent anticancer activities, such as anti-proliferation and pro-apoptotic activities against cancer cells. Furthermore, the expression of the apoptosis-inducing proteins, p53 and caspase-3, significantly increased in response to ETBO, whereas the expression of the anti-apoptotic protein, Bcl-2, decreased. These data imply that ETBO induces apoptosis by caspase activation through the modulation of pro-apoptotic and anti-apoptotic gene, p53 and Bcl-2, respectively. In addition, ETBO significantly inhibited migration and invasion of cervical cancer cells in a concentration-dependent manner.ConclusionIn this study, ETBO exhibited considerable anticancer activities, such as inhibition of proliferation, invasion, and migration, as well as induction of apoptosis. These data suggest that ETBO is a promising therapeutic agent in cancer therapy and drug discovery.

【 授权许可】

CC BY   
© The Author(s). 2017