Screening for bioactivity of Mutinus elegans extracts

14th International Conference on Science, Engineering and Technology

自然科学;工业技术

Gajendiran, A.^1 ; Cyriac, R.E.^1 ; Abraham, J.^1

Microbial Biotechnology Laboratory, School of Biosciences and Technology, VIT University, Vellore, Tamil Nadu

632014, India^1

关键词: Anti-microbial activity;    Anticancer activities;    Antimicrobial and antioxidant activities;    Bioactive compounds;    Cytotoxicity assays;    Methanol solvent system;    Microbiological assays;    Zone of inhibitions;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/263/2/022039/pdf
DOI  :  10.1088/1757-899X/263/2/022039

来源: IOP

【 摘 要 】

Mutinus elegans is a species of fungi that is commonly called as Elegant Stinkhorn. The aim of this study was to screen the crude extracts of the fungus for phytochemical analysis, antimicrobial activity, antioxidant assay and anticancer activity. Extraction of the fungal sample in Soxhlet apparatus was done with n-hexane and methanol as the solvent. Stock solutions of the crude methanol extract were prepared and used for microbiological assay. Thin layer chromatography was performed in order to determine the number of active components in n-hexane, and methanol solvent system for the fungus Mutinus elegans. Further, antioxidant assay was performed using DPPH radical scavenging assay. The fungal sample was then tested for cytotoxicity assay against MG63 osteosarcoma cell lines. The antimicrobial assay of Mutinus elegans extract exhibited activity against five pathogens. The zone of inhibition was measured with respect to standard antibiotics. Gas chromatography and Mass spectrometry (GC/MS analysis), revealed the presence of dibromo-tetradecan-1-ol-acetate, 2-myristynoyl-glycinamide, fumaric acid, and cyclohexylmethyldecyl ester compounds were presented in methanol and n-hexane extract of Mutinus elegans. The present study concludes the presence of bioactive compound in the extract which exhibited antimicrobial and antioxidant activity in Mutinus elegans.