Alterations in B-cell subsets in pediatric patients with early atopic dermatitis

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:140

Article

Czarnowicki, Tali^1,3,4  Esaki, Hitokazu^1,3,4  Gonzalez, Juana²  Renert-Yuval, Yael^3,4  Brunner, Patrick¹  Oliva, Margeaux^3,4  Estrada, Yeriel^3,4  Xu, Hui^3,4  Zheng, Xiuzhong¹  Talasila, Sreya^7,8  Haugh, Isabel^7,8  Thy Huynh^7,8  Lyon, Sarah^7,8  Tran, Gary^7,8  Sampson, Hugh⁵  Suarez-Farinas, Mayte^3,4,6,9  Krueger, James G.¹  Guttman-Yassky, Emma^1,3,4  Paller, Amy S.^7,8 

[1] Rockefeller Univ, Lab Invest Dermatol, 1230 York Ave, New York, NY 10065 USA

[2] Rockefeller Univ, Translat Technol Core Lab, 1230 York Ave, New York, NY 10021 USA

[3] Icahn Sch Med Mt Sinai, Dept Dermatol, New York, NY 10029 USA

[4] Icahn Sch Med Mt Sinai, Lab Inflammatory Skin Dis, New York, NY 10029 USA

[5] Icahn Sch Med Mt Sinai, Jaffe Food Allergy Inst, New York, NY 10029 USA

[6] Icahn Sch Med Mt Sinai, Dept Populat Hlth Sci & Policy, New York, NY 10029 USA

[7] Northwestern Univ, Dept Dermatol, Feinberg Sch Med, Chicago, IL 60611 USA

[8] Northwestern Univ, Dept Pediat, Feinberg Sch Med, Chicago, IL 60611 USA

[9] Icahn Inst Genom & Multiscale Biol, Dept Genet & Genom Sci, New York, NY USA

关键词: Atopic dermatitis;    B cells;    T cells;    allergen-specific IgE;    atopic march;   

DOI  :  10.1016/j.jaci.2016.09.060

来源: Elsevier

【 摘 要 】

Background: B cells undergo maturation and class-switching in response to antigen exposure and T-cell help. Early B-cell differentiation has not been defined in patients with early-onset atopic dermatitis (AD). Objective: We sought to define the frequency of B-cell subsets associated with progressive B-cell maturation and IgE class-switching. Methods: We studied 27 children and 34 adults with moderate-to-severe AD (mean SCORAD score, 55 and 65, respectively) and age-matched control subjects (15 children and 27 adults). IgD/CD27 and CD24/CD38 core gating systems and an 11-color flow cytometric panel were used to determine the frequencies of circulating B-cell subsets. Serum total and allergen-specific IgE (sIgE) levels were measured by using ImmunoCAP. Results: Compared with adults, children showed T-cell predominance in the skin. Circulating CD19(+) CD20(+) B-cell counts were lower in patients with pediatric AD than in control subjects (24% vs 33%, P = .04), whereas CD3(+) T-cell counts were higher (62% vs 52%, P = .05). A decreased B-cell/T-cell lymphocyte ratio with age was observed only in pediatric control subjects (r = -0.48, P = .07). In pediatric patients with AD, a positive correlation was observed between B-cell/T-cell ratio and nonswitched memory B-cell counts (r = 0.42, P = .03). Higher frequencies of positive sIgE levels were seen in pediatric patients with AD (P < .0001). Diverse sIgE levels correlated with SCORAD scores and age of pediatric patients with AD (P < .01). Positive correlations were observed between activated B-cell and memory T-cell counts (P < .02). In patients with AD, IgE sensitization to most allergens clustered with age, T(H)1, T(H)2, total IgE levels, and B-cell memory subsets. Conclusions: Peripheral B and T cells are altered in pediatric patients with early AD, but T cells predominate in skin lesions.

【 授权许可】

Free