집먼지 진드기 추출물이 사람 각질 세포주인 HaCaT에서 TARC생성과 IL-22수용체 발현 변화에 미치는 영향

Atopic dermatitis;HDM extract;HaCaT;IL-22;T cells;610

의과대학 의학과 ;

University:서울대학교 대학원

关键词: Atopic dermatitis;    HDM extract;    HaCaT;    IL-22;    T cells;    610;   

Others  :  http://s-space.snu.ac.kr/bitstream/10371/132793/1/000000067122.pdf

美国|英语

来源: Seoul National University Open Repository

【 摘 要 】

House dust mite (HDM) is known for one of factors that causes atopic dermatitis (AD). HDM aggravated symptoms of AD patient via the production of tumor necrosis factor (TNF)-α and interferon (IFN)-γ from keratinocyte. It is known that thymus and activation regulated chemokine (TARC) is highly expressed in AD lesion to recruit CCR4 expressing T cells. Interleukin (IL)-22, a member of IL-10 family, is produced by CD4+ T cell, natural killer (NK) cell and NKT cell. Its receptor is a heterodimer of IL-22Rα and IL-10Rβ. It is expressed on skin, pancreas, kidney and epithelial cells. In the skin of chronic AD patients, IL-22 is increased and shows pro-inflammatory effects. However, the effect of HDM on IL-22 production from T cells and on TARC production and IL-22Rα receptor expression in keratinocyte are largely unknown. Therefore, I investigated IL-22Rα expression and TARC production in human keratinocyte cell line, HaCaT by the treatment of HDM extract and their role in HDM-induced skin inflammation. As a result, HDM extract increased IL-22Rα expression and TARC production in HaCaT, although HDM extract could not remarkably increase of the proliferation of HaCaT. HDM extract enhanced TNF-α and IFN-γ production from T cells and it caused the increase TARC production from T cells. In addition, HDM extract increased IL-22 from T cells. Interestingly, I found that the production of IL-1α, IL-6 and TARC from HaCaT, when they were treated with rIL-22 or culture supernatant of HDM extract-treated T cells. It suggests that HDM extract induces the increase of IL-22 production from T cells, and then it produces IL-1α, IL-6 and TARC from HaCaT. Finally, I examined whether T cell migration is facilitated by culture supernatant of HDM extract-treated HaCaT, because I have already confirmed that TARC production is increased from HaCaT by HDM extract treatment. As I expected, T cell migration is increased by culture supernatant of HDM extract-treated HaCaT. Taken together, HDM extract not only increases IL-22Rα expression and TARC production in HaCaT, but also increases IL-22 production from T cells. And then, TARC production from HaCaT via the interaction between IL-22 and IL-22Rα facilitates T cell migration. And it might be one of the reason for inflammation in the skin lesion of AD patients.