Modulation of experimental atopic dermatitis by topical application of Gami-Cheongyeul-Sodok-Eum

BMC Complementary and Alternative Medicine

Research Article

Young-Beob Yu¹  Jung-Eun Kim²  Ji Sun Hwang²  Sin-Hyeog Im² 

[1] Department of Herbal Pharmaceutical Development, Nambu University, 23, Cheomdan-jungangro, 506-706, Gwangsan-gu, Gwangju, Republic of Korea;School of Life Sciences and Immune Synapse Research Center, Gwangju Institute of Science and Technology (GIST), 500-712, Gwangju, Republic of Korea;

关键词: Atopic dermatitis;    Herbal medicine;    GCSE (Gami-Cheongyeul-Sodok-Eum);    Th1 & Th2 cells;    B cells;    IgE and Cytokines;   

DOI  :  10.1186/1472-6882-13-312

 received in 2013-10-26, accepted in 2013-11-01,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundGami-Cheongyeul-Sodok-Eum (GCSE), an herbal formula of traditional Korean medicine, comprises nine herb components. GCSE has various biological activities such as anti-inflammatory, anti-bacterial and anti-viral activities. However, it is still unclear whether GCSE has any immunomodulatory effect on atopic dermatitis (AD).MethodsGCSE was treated to primary B cells and CD4+ T cells isolated from atopic mice to compare its inhibitory effects on IgE secretion and cytokine expression. Experimental AD was established by alternative treatment of 2, 4-dinitrochlorobenzene (DNCB) and house dust mite extract to the ears of BALB/c mice. GCSE was topically applied to ears of atopic mice every day for 3 weeks. AD progression was analyzed by measuring ear thickness, serum IgE level, histological examination of ear tissue by H&E staining and cytokine profile of CD4+ T cells and CD19+ B cells by real time PCR and ELISA.ResultsTreatment of GCSE significantly reduced IgE production and expression of AD associated pathogenic cytokines such as IL-4, IL-5, IL-10, IL-13, IL-17, TNF-α, and IFN-γ by lymphocytes isolated from AD-induced mice. Topical application of GCSE on the ears of AD-induced mice significantly reduced ear thickness, clinical score and lymphocytes infiltration to ears as compared to control group. GCSE treatment also reduced serum IgE level and the levels of major pathogenic cytokines such as IL-4, IL-5, IL-10, IL-13 and IL-17. In addition, GCSE treatment significantly increased Foxp3 expression level.ConclusionsThe protective effect of GCSE in experimental AD is mediated by inhibition of IgE production, by reduction in the levels of pathogenic cytokines and by induction of Foxp3, all of which are suggesting the beneficial effect of GCSE on modulating atopic dermatitis.

【 授权许可】

Unknown   
© Hwang et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.