Integrated platform for detecting pathogenic DNA via magnetic tunneling junction-based biosensors

SENSORS AND ACTUATORS B-CHEMICAL

卷:242

Article

Sharma, Parikshit P.¹  Albisetti, Edoardo¹  Massetti, Matteo¹  Scolari, Martina¹  La Torre, Chiara¹  Monticelli, Marco¹  Leone, Marco²  Damin, Francesco³  Gervasoni, Giacomo⁴  Ferrari, Giorgio⁴  Salice, Fabio⁵  Cerquaglia, Emanuele⁶  Falduti, Giorgio⁷  Cretich, Marina³  Marchisio, Edoardo⁷  Chiari, Marcella³  Sampietro, Marco⁴  Petti, Daniela¹  Bertacco, Riccardo¹ 

[1] Politecn Milan, Dipartimento Fis, Via Giuseppe Colombo 81, I-20133 Milan, Italy

[2] Politecn Milan, Dipartimento Fis, L NESS, Via Anzani 42, I-22100 Como, Italy

[3] CNR, Ist Chim Riconoscimento Mol, Via Mario Bianco 9, I-20131 Milan, Italy

[4] Politecn Milan, Dipartimento Elettron Informaz & Bioingn, Via Giuseppe Colombo 81, I-20133 Milan, Italy

[5] Politecn Milan, Dipartimento Elettron Informaz & Bioingn, Via Anzani 42, I-22100 Como, Italy

[6] Myti Tecnol Informat Srl, Via Branze 44, I-25123 Brescia, Italy

[7] Diapro Diagnost Bioprobes Srl, Via Giosue Carducci 27, I-20099 Sesto San Giovanni, Italy

关键词: Magnetic biosensor;    Magnetic tunneling junction;    Magnetic bead;    Genotyping;    Listeria;    Point-of-care;   

DOI  :  10.1016/j.snb.2016.11.051

来源: Elsevier

【 摘 要 】

In recent years, the development of portable platforms for performing fast and point-of-care analyses has drawn considerable attention for their wide variety of applications in life science. In this framework, tools combining magnetoresistive biosensors with magnetic markers have been widely studied in order to detect concentrations of specific molecules, demonstrating high sensitivity and ease of integration with conventional electronics. In this work, first, we develop a protocol for efficient hybridization of natural DNA; then, we show the detection of hybridization events involving natural DNA, namely genomic DNA extracted from the pathogenic bacterium Listeria monocytogenes, via a compact magnetic tunneling junction (MTJ)-based biosensing apparatus. The platform comprises dedicated portable electronic and microfluidic setups, enabling point-of-care biological assays. A sensitivity below the nM range is demonstrated. This work constitutes a step forward towards the development of portable lab-on-chip platforms, for the multiplexed detection of pathogenic health threats in food and food processing environment. (C) 2016 Elsevier B.V. All rights reserved.

【 授权许可】

Free