Use of Alternative Primers for Gender Discrimination in Human Forensic Genotyping

Kupfer DM, Jenkins M, Burian D, Canfield DV

FAA Office of Aerospace Medicine - Civil Aerospace Medical Institute

关键词: PCR;    Genotyping;    Gender Determination;    Electrophoresis;   

RP-ID  :  DOT/FAA/AM-08/8

美国|英语

来源: Federal Aviation Administration

【 摘 要 】

An assay using the Federal Bureau of Investigation�s human Combined DNA Identity System (CODIS) primers has been developed for polymerase chain reaction (PCR)-based human identity testing. Recent forensic literature has identified several human populations that carry a deletion mutation in the Y-chromosome copy of the amelogenin locus. This is the standard locus used for gender determination in CODIS. Additionally, the amelogenin male PCR products are very close in size requiring manual annotation of PCR electrophoresis results for this locus.