A fully automated 384 capillary array for DNA sequencer. Final report

Li, Qingbo ; Kane, T

SpectruMedix (United States)

关键词: Dna;    Computers;    59 Basic Biological Sciences;    Commercialization;    Electrophoresis;   

DOI  :  10.2172/819022
RP-ID  :  Final Report
RP-ID  :  FG02-97ER82461
RP-ID  :  819022

美国|英语

来源: UNT Digital Library

【 摘 要 】

Phase I SpectruMedix has successfully developed an automatic 96-capillary array DNA prototype based on the multiplexed capillary electrophoresis system originated from Ames Laboratory-USDOE, Iowa State University. With computer control of all steps involved in a 96-capillary array running cycle, the prototype instrument (the SCE9600) is now capable of sequencing 450 base pairs (bp) per capillary, or 48,000 bp per instrument run within 2 hrs. Phase II of this grant involved the advancement of the core 96 capillary technologies, as well as designing a high density 384 capillary prototype. True commercialization of the 96 capillary instrument involved finalization of the gel matrix, streamlining the instrument hardware, creating a more reliable capillary cartridge, and further advancement of the data processing software. Together these silos of technology create a truly commercializable product (the SCE9610) capable of meeting the operation needs of the sequencing centers.