The first evaluation of glucose-6-phosphate dehydrogenase deficiency (G6PD) gene mutation in malaria-endemic region at South Central Timor (SCT) district, Eastern Indonesia 2015-2016

1st International Conference on Tropical Medicine and Infectious Diseases (ICTROMI) Faculty of Medicine Universitas Sumatera Utara

Hutagalung, J.^1 ; Kusnanto, H.^2 ; Supargiyono^3 ; Sadewa, A.H.^4 ; Satyagraha, A.W.^5

Center for Tropical Medicine, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia^1

Field Epidemiology Training Program, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia^2

Department of Parasitology, Entomology and Tropical Disease University Gadjah Mada, Yogyakarta, Indonesia^3

Department of Biochemistry, Faculty of Medicine, University Gadjah Mada, Yogyakarta, Indonesia^4

Eijkman Institute for Molecular Biology, Red Blood Cell and Enzyme Disorder Laboratories, Jakarta, Indonesia^5

关键词: Blood samples;    DNA Sequencing;    Gene mutations;    Genotyping;    Glucose-6-phosphate dehydrogenase;    Indonesia;    PCR-RFLP;    Quantitative tests;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/125/1/012016/pdf
DOI  :  10.1088/1755-1315/125/1/012016

来源: IOP

【 摘 要 】

Primaquine (PQ) is the only licensed drug effective against P. vivax for specific hypnozoites and as a key drug in the malaria elimination stage. However, PQ can cause severe hemolysis in G6PD deficient individuals. Unfortunately, few epidemiological data of these disorders was in Indonesia. This study aimed to assesses the prevalence and genotyping variant of G6PDd among the people on malaria-endemic. Blood samples from 555 unrelated subjects in eastern Indonesia were for G6PDd by quantitative test and PCR-RFLP-DNA sequencing. All protocols followed by Promega, Madison, USA. The prevalence of malaria and anemia was 32.6% (181/555) and 16% (89/555) with P. vivaxdominant species 52.5% (95/181), respectively. Overall, 16.6% (92/555) subjects were G6PD deficient, including 58.7% (54/92) females and 41.3% (38/92). Among the 92 cases G6PD deficient molecularly studied, the genotype variant Vanua Lava (T10883C) were detected dominant and unknown G6PD deficient (T-13.154-C) in 3 cases. It was high G6PD deficient in eastern Indonesia indicate that diagnosis and management of G6PD deficient are necessary. Obligatory anti-malaria doses for G6PD deficient individuals, population screening, are needed on endemic malaria in eastern Indonesia.