Formation of MspA channel on Nanopore-Spanning Lipid Bilayer

2018 5th International Conference on Advanced Composite Materials and Manufacturing Engineering

Cai, Lingfeng^1 ; Zhu, Libo^1 ; Liu, Quanjun^1

School of Biological Science and Medical Engineering, Southeast University, Nanjing, China^1

关键词: DNA Sequencing;    Enhanced stability;    Formation process;    High throughput;    Lipid bilayer membranes;    Nanopore array;    Protein functions;    Single molecule;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/394/2/022067/pdf
DOI  :  10.1088/1757-899X/394/2/022067

来源: IOP

【 摘 要 】

Biological nanopores are novel and versatile single-molecule sensor for individual label-free biopolymer detection and characterization. However, their studies and applications require a stable lipid bilayer to maintain protein function. Herein, we provided a method for producing lipid bilayers across nanopore array on a silicon nitride substrate by vesicle technique and MspA was embedded into lipid bilayer membrane formed ion channel. The formation process and stability of lipid bilayer membrane and translocation of samples were monitored real-time by patch clamp. The hybrid nanopore system exhibit excellent electrical properties and enhanced stability, making this system ideal for high-throughput DNA sequencing.