Testing MiniSTR Primers for Addition to a PCR-Based Forensic Specimen Identification Protocol

Guzman L, Kupfer DM, Burian D

FAA Office of Aerospace Medicine - Civil Aerospace Medical Institute

关键词: Genotyping;    Polymerase Chain Reaction;    miniSTR;   

RP-ID  :  DOT/FAA/AM-09/21

美国|英语

来源: Federal Aviation Administration

【 摘 要 】

An assay has been developed for polymerase chain reaction (PCR)-based human identity testing using the Federal Bureau of Investigation�s human Combined DNA Identity System (CODIS) primers. Recent forensic literature has identified difficulties using these primers due to amplicon size and the degraded nature of DNA from forensic samples. Primers termed mini Short-Tandem Repeat (STR) primers targeted to the same loci as the CODIS primers but which have smaller amplicons have been developed.