Variant discovery in the sheepmeat odour and flavour in javanese fat tailed sheep using RNA sequencing

1st International Conference on Food Security and Sustainable Agriculture in The Tropics

农业科学;经济学

Abuzahra, M.A.M.^1 ; Jakaria^1 ; Listyarini, K.^1 ; Furqon, A.^1 ; Sumantri, C.^1 ; Uddin, M.J.^2 ; Gunawan, A.^1

Department of Animal Production and Technology, Faculty of Animal Science, Bogor Agricultural University, Bogor

16680, Indonesia^1

School of Veterinary Science, University of Queensland, Gatton Campus, Gatton

QLD

4343, Australia^2

关键词: Association analysis;    Genome assembly;    Genotyping;    High throughput;    Meat production;    Meat samples;    Rna sequencing;    Transcriptomes;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/157/1/012030/pdf
DOI  :  10.1088/1755-1315/157/1/012030

学科分类：农业科学（综合）

来源: IOP

【 摘 要 】

High-throughput RNA sequencing (RNA-Seq) reveals new challenges for the detection of transcriptome variants (SNPs) in different tissues and species. The aims of this study was to characterize a SNP discovery analysis in the sheep meat odour and flavour transcriptome using RNA-Seq. Six liver samples from divergent sheep meat odour and flavour were analyzed using the Illumina Genome Hiseq 2500 Analyzer. The SNP detection analysis revealed 142 SNPs in sheep meat samples, and a large number of those corresponded to differences between high and low sheep meat odour and flavour ovis genome assembly OAR v4.0. Among them, about 90.4% of genes had multiple polymorphisms within 12 genes (JAML, ANGPTL8, LOC101103463, SEPW1, SCN5A, LOC101113036, DOCK6, GTSE1, KIF12, KCTD17, KANK2, CYP2A6). Several of the SNPs (JAML, CYP2A6, SEPW1, and KIF12) found in this study could be included as suitable markers in genotyping platforms to perform association analyses in commercial populations and apply genomic selection protocols in the sheep meat production.