Association Study between Phospholipid Profile and SNPs Related to Fatty Acid Metabolism in Hanwoo Beef

Fatty acid;Omega-3;Omega-6;Beef;SNP;Association analysis;631

국제농업기술대학원 국제농업기술학과 ;

University:서울대학교 대학원

关键词: Fatty acid;    Omega-3;    Omega-6;    Beef;    SNP;    Association analysis;    631;   

Others  :  http://s-space.snu.ac.kr/bitstream/10371/127128/1/000000141008.pdf

美国|英语

来源: Seoul National University Open Repository

【 摘 要 】

FASN (rs41919985), SCD (rs41255693), FABP4 (rs41729173), FADS1 (rs136261927 and rs42187261) and FADS2 (rs109772589).1. Total lipid was extracted using Folch method, and phospholipid was separated from total lipid using thin-layer chromatography. Phospholipid fraction was scraped from TLC plate and directly methylated using O;;Fallon’s direct methylation method. This preparation method was validated by comparing with O;;Fallon’s direct methylation method and analyzing gas chromatography data of empty silica through the preparation method. The same amount of phosphatidylcholine from egg yolk was treated by above preparation method or direct methylation without pretreatment, and GC data from each method were compared. Total areas through the preparation method or direct methylation were comparable having 407 and 470, respectively. 2. Fatty acid composition in phospholipid of the samples were analyzed with GC. Genomic DNA was extracted from the fifty-six samples and genotyped for the candidate SNPs using restriction fragment length polymorphism or Sanger sequencing. In the case of rs109772589 in FADS2, all the samples showed the same hetero genotype, and thus it was omitted from the association analysis. On the other hand, in the case of rs41919985 in FASN, there were only two genotypes except AA type. 3. Associations between omega fatty acid composition and genotypes were analyzed by ANOVA. There were four SNPs showing statistically significant associations.GA type of rs41919985 with the highest C20:5 n-3 (p=0.027) CC type of rs41729173 with the lowest C22:2n-6 (p=0.049) AG type of rs42187261 with the lowest C20:4 n-6 (p=0.044)GA type of rs136261927 with the lowest C20:3n-6 (p=0.027)The ratio of total n-3/n-6 of the individual which have GA, CC, AG and GA types in rs41919985, rs41729173, rs42187261 and rs136261927, respectively, was around 28:1. This value was the third highest grade in total n-3/n-6 ratio of the samples and much higher than the mean value of total n-3/n-6 ratio. Consequently, these four SNPs could be applied as potential genetic markers to select Hanwoo steers in the aspect of improvement of n-3/n-6 balance in the future.