Population structure and spatio-temporal transmission dynamics of Plasmodium vivax after radical cure treatment in a rural village of the Peruvian Amazon

Malaria Journal

Research

Umberto D’Alessandro¹  Veronica E Soto-Calle²  Alejandro Llanos-Cuentas²  Angel Rosas³  Dionicia Gamboa⁴  Peter Van den Eede⁵  Annette Erhart⁵  Hugo Rodriguez Ferrucci⁶  Jean-Pierre Van Geertruyden⁷  Christopher Delgado-Ratto⁸  Emmanuel N Abatih⁹ 

[1] Department of Public Health, Institute of Tropical Medicine, B-2000, Nationalestraat 155, Antwerp, Belgium;Medical Research Council Unit, Fajara, PO Box 273, Banjul, The Gambia;Grupo de Estudio de Leishmaniasis y Malaria (GELM), ITMAvH-Universidad Peruana Cayetano Heredia (UPCH), Av Honorio Delgado 430, Lima 31, Peru;Grupo de Estudio de Leishmaniasis y Malaria (GELM), ITMAvH-Universidad Peruana Cayetano Heredia (UPCH), Av Honorio Delgado 430, Lima 31, Peru;Malariology Unit, Department of Biomedical Sciences, Institute of Tropical Medicine, B-2000, Antwerp, Nationalestraat 155, Antwerp, Belgium;Laboratory of Malaria, Unit of Molecular Epidemiology, Institute of Tropical Medicine Alexander von Humboldt (IMTAvH)-Universidad Peruana Cayetano Heredia (UPCH), Av Honorio Delgado 430, Lima, Peru;Departamento de Ciencias Celulares y Moleculares, Facultad de Ciencias y Filosofía- Universidad Peruana Cayetano Heredia, Av Honorio Delgado 430, Lima, Peru;Malariology Unit, Department of Biomedical Sciences, Institute of Tropical Medicine, B-2000, Antwerp, Nationalestraat 155, Antwerp, Belgium;Ministry of Health, Av 28 de Julio s/n, Punchana, Loreto, Peru;Unit of International Health, ESOC Department, Faculty of Medicine, University of Antwerp, Universiteitsplein 1, B-2610, Antwerp, Belgium;Unit of International Health, ESOC Department, Faculty of Medicine, University of Antwerp, Universiteitsplein 1, B-2610, Antwerp, Belgium;Laboratory of Malaria, Unit of Molecular Epidemiology, Institute of Tropical Medicine Alexander von Humboldt (IMTAvH)-Universidad Peruana Cayetano Heredia (UPCH), Av Honorio Delgado 430, Lima, Peru;Unit of Veterinary Biostatistics and Epidemiology, Institute of Tropical Medicine, B-2000, Nationalestraat 155, Antwerp, Belgium;

关键词: Plasmodium vivax;    Malaria;    Genotyping;    Microsatellites;    Population genetics;    Spatio-temporal analysis;    Peruvian amazon;   

DOI  :  10.1186/1475-2875-13-8

 received in 2013-09-04, accepted in 2013-12-28,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundDespite the large burden of Plasmodium vivax, little is known about its transmission dynamics. This study explored the population structure and spatio-temporal dynamics of P. vivax recurrent infections after radical cure in a two-year cohort study carried out in a rural community of the Peruvian Amazon.MethodsA total of 37 P. vivax participants recruited in San Carlos community (Peru) between April and December 2008 were treated radically with chloroquine and primaquine and followed up monthly for two years with systematic blood sampling. All samples were screened for malaria parasites and subsequently all P. vivax infections genotyped using 15 microsatellites. Parasite population structure and dynamics were determined by computing different genetic indices and using spatio-temporal statistics.ResultsAfter radical cure, 76% of the study participants experienced one or more recurrent P. vivax infections, most of them sub-patent and asymptomatic. The parasite population displayed limited genetic diversity (He = 0.49) and clonal structure, with most infections (84%) being monoclonal. Spatio-temporal clusters of specific haplotypes were found throughout the study and persistence of highly frequent haplotypes were observed over several months within the same participants/households.ConclusionsIn San Carlos community, P. vivax recurrences were commonly observed after radical treatment, and characterized by asymptomatic, sub-patent and clustered infections (within and between individuals from a few neighbouring households). Moreover low genetic diversity as well as parasite inbreeding are likely to define a clonal parasite population which has important implications on the malaria epidemiology of the study area.

【 授权许可】

CC BY   
© Delgado-Ratto et al.; licensee BioMed Central Ltd. 2014