Global and local genetic diversity at two microsatellite loci in Plasmodium vivax parasites from Asia, Africa and South America

Malaria Journal

Research

Ib C Bygbjerg¹  Michael Alifrangis¹  Samir Ranjitkar¹  Flemming Konradsen¹  Mette L Schousboe¹  Rupika S Rajakaruna²  Rosalynn Ord³  Nahla Gadalla³  Mark Rowland³  Toby Leslie³  Richard Pearce³  Cally Roper³  Priyanie H Amerasinghe⁴  Francisco Morales⁵ 

[1] Centre for Medical Parasitology, Institute of International Health, Immunology, and Microbiology, University of Copenhagen, Copenhagen, Denmark;Department of Infectious Diseases, Copenhagen University Hospital, Copenhagen, Denmark;Department of Zoology, University of Peradeniya, 20400, Peradeniya, Sri Lanka;Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, WC1 4HT, London, UK;International Water Management Institute, Hyderabad, Andhra Pradesh, India;Robles Y Pampite, Cymbaya, Universidad San Francisco de Quito, San Francisco de Quito, Ecuador;

关键词: Malaria;    Plasmodium vivax;    Genotyping;    Microsatellites;    Diversity;    Heterozygosity;   

DOI  :  10.1186/1475-2875-13-392

 received in 2014-05-09, accepted in 2014-08-28,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundEven though Plasmodium vivax has the widest worldwide distribution of the human malaria species and imposes a serious impact on global public health, the investigation of genetic diversity in this species has been limited in comparison to Plasmodium falciparum. Markers of genetic diversity are vital to the evaluation of drug and vaccine efficacy, tracking of P. vivax outbreaks, and assessing geographical differentiation between parasite populations.MethodsThe genetic diversity of eight P. vivax populations (n = 543) was investigated by using two microsatellites (MS), m1501 and m3502, chosen because of their seven and eight base-pair (bp) repeat lengths, respectively. These were compared with published data of the same loci from six other P. vivax populations.ResultsIn total, 1,440 P. vivax samples from 14 countries on three continents were compared. There was highest heterozygosity within Asian populations, where expected heterozygosity (He) was 0.92-0.98, and alleles with a high repeat number were more common. Pairwise FST revealed significant differentiation between most P. vivax populations, with the highest divergence found between Asian and South American populations, yet the majority of the diversity (~89%) was found to exist within rather than between populations.ConclusionsThe MS markers used were informative in both global and local P. vivax population comparisons and their seven and eight bp repeat length facilitated population comparison using data from independent studies. A complex spatial pattern of MS polymorphisms among global P. vivax populations was observed which has potential utility in future epidemiological studies of the P. vivax parasite.

【 授权许可】

Unknown   
© Schousboe et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.