Characterization of interaction of magnetic nanoparticles with breast cancer cells

Journal of Nanobiotechnology

Research

Macarena Calero¹  Angeles Villanueva¹  Ana Lazaro-Carrillo¹  Kieran Crosbie-Staunton²  Adriele Prina-Mello³  Yuri Volkov³  Michele Chiappi⁴  María José Rodríguez⁴  Francisco Javier Chichón⁴  José L Carrascosa⁵ 

[1] Departamento de Biología, Universidad Autónoma de Madrid, Cantoblanco, 28049, Madrid, Spain;Instituto Madrileño de Estudios Avanzados en Nanociencia (IMDEA Nanociencia), Cantoblanco, 28049, Madrid, Spain;Department of Clinical Medicine, Trinity Centre for Health Science, James’s Street, 8, Dublin, Ireland;Department of Clinical Medicine, Trinity Centre for Health Science, James’s Street, 8, Dublin, Ireland;Centre for Research on Adaptive Nanostructures and Nanodevices (CRANN), and AMBER Centre, Trinity College Dublin, College Green, 2, Dublin, Ireland;Department of Macromolecular Structure, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, 28049, Madrid, Spain;Department of Macromolecular Structure, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, 28049, Madrid, Spain;Instituto Madrileño de Estudios Avanzados en Nanociencia (IMDEA Nanociencia), Cantoblanco, 28049, Madrid, Spain;

关键词: MCF-7 cells;    Superparamagnetic iron oxide nanoparticles;    Intracellular trafficking;    Transmission electron microscopy;    Cellular uptake;    Endocytosis;    Cytotoxicity;   

DOI  :  10.1186/s12951-015-0073-9

 received in 2014-10-03, accepted in 2015-01-28,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundDifferent superparamagnetic iron oxide nanoparticles have been tested for their potential use in cancer treatment, as they enter into cells with high effectiveness, do not induce cytotoxicity, and are retained for relatively long periods of time inside the cells. We have analyzed the interaction, internalization and biocompatibility of dimercaptosuccinic acid-coated superparamagnetic iron oxide nanoparticles with an average diameter of 15 nm and negative surface charge in MCF-7 breast cancer cells.ResultsCells were incubated with dimercaptosuccinic acid-coated superparamagnetic iron oxide nanoparticles for different time intervals, ranging from 0.5 to 72 h. These nanoparticles showed efficient internalization and relatively slow clearance. Time-dependent uptake studies demonstrated the maximum accumulation of dimercaptosuccinic acid-coated superparamagnetic iron oxide nanoparticles after 24 h of incubation, and afterwards they were slowly removed from cells. Superparamagnetic iron oxide nanoparticles were internalized by energy dependent endocytosis and localized in endosomes. Transmission electron microscopy studies showed macropinocytosis uptake and clathrin-mediated internalization depending on the nanoparticles aggregate size. MCF-7 cells accumulated these nanoparticles without any significant effect on cell morphology, cytoskeleton organization, cell cycle distribution, reactive oxygen species generation and cell viability, showing a similar behavior to untreated control cells.ConclusionsAll these findings indicate that dimercaptosuccinic acid-coated superparamagnetic iron oxide nanoparticles have excellent properties in terms of efficiency and biocompatibility for application to target breast cancer cells.

【 授权许可】

Unknown   
© Calero et al.; licensee BioMed Central. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.