Biological activity and magnetic resonance imaging of superparamagnetic iron oxide nanoparticles-labeled adipose-derived stem cells

Stem Cell Research & Therapy

Minming Zhang¹  Risheng Yu¹  Xinying Wu¹  Liyong Jie¹  Yanbin Tan¹  Jingjing Fan¹ 

[1] Department of Radiology, 2nd Affiliated Hospital, Medical College, Zhejiang University, 88 Jiefang Road, Hangzhou 310009, China

关键词: Magnetic resonance imaging;    Superparamagnetic iron oxide nanoparticles;    Bone marrow mesenchymal stem cells;    Adipose-derived stem cells;   

Others  :  847570
DOI  :  10.1186/scrt191

 received in 2012-11-17, accepted in 2013-04-12,  发布年份 2013

【 摘 要 】

Introduction

No comparative study of adipose-derived stem cells (ADSCs) and bone marrow mesenchymal stem cells (BMSCs) by using superparamagnetic iron oxide nanoparticles (SPIOs)-labeling and magnetic resonance imaging (MRI) has been performed.

Methods

We studied the biological activity and MRI of ADSCs by labeling them with SPIOs and comparing them with BMSCs. After incubating the cells in culture medium with different levels of SPIOs (control group: 0 μg/ml; Groups 1 to 3: 25, 50, and 100 μg/ml) for 24 hours, we compared ADSCs with BMSCs in terms of intracellular iron content, labeling efficiency, and cell viability. Stem cells in the culture medium containing 50 μg/ml SPIOs were induced into osteoblasts and fat cells. Adipogenic and osteogenic differentiation potentials were compared. R₂* values of MRI in vitro were compared.

Results

The results showed that labeling efficiency was highest in Group 2. Intracellular iron content and R₂* values increased with increasing concentrations of SPIOs, whereas cell viability decreased with increasing concentrations of SPIOs, and adipogenic and osteogenic differentiation potentials decreased. However, we found no significant difference between the two kinds of cells for any of these indexes.

Conclusions

ADSCs can be labeled and traced as easily as BMSCs in vitro. Given their abundance and higher proliferative capacity, as was previously shown, ADSCs may be better suited to stem cell therapy than are BMSCs.

【 授权许可】

   
2013 Fan et al.; licensee BioMed Central Ltd.

【 图 表 】

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