Dislocation structures in a nearly single phase annealed Mo5SiB2 T2 alloy have been investigated by transmission electron microscopy (TEM). The dislocations have been subjected to Burgers vector and trace analyses to determine the slip directions and planes with the aid of image simulations generated using single crystal elastic constants derived from first principles calculations. The experimental results ate compared to predicted slip directions and planes from anisotropic eleasticilty calculations. Thermal expension coefficients have been measured by dilatometry and are compared to both calculated and previous experimental values measured using diffraction techniques. Lastly, preliminary compression testing has been performed on the single phase material at 1200 C.