GE11 peptide modified CSO-SPION micelles for MRI diagnosis of targeted hepatic carcinoma

Biotechnology & Biotechnological Equipment

Guang-Hai Yan¹  Ji-Shan Quan²  Zhuo-Yan Liu³  Yu-Zhu Guo³  Kai-Xuan Xu³  Guang-Yu Jin³  Xiao-Yu Li³  Zhuo Zhang³ 

[1] Department of Anatomy, Basic Medical College, Yanbian University, Yanji, Jilin, PR Chin;Department of Pharmacy, College of Pharmacy, Yanbian University, Yanji, Jilin, PR Chin;Department of Radiology, Affiliated Hospital of Yanbian University, Yanji, Jilin, PR Chin;

关键词: Superparamagnetic iron oxide nanoparticles;    epidermal growth factor receptor;    hepatocellular carcinoma;    magnetic resonance imaging;   

DOI  :  10.1080/13102818.2021.1997154

来源: Taylor & Francis

【 摘 要 】

This study investigated the performance of GE11 peptide-modified superparamagnetic iron oxide nanoparticles (SPION) targeting the epidermal growth factor receptor (EGFR) on the surface of hepatocellular carcinoma (HCC) cells for magnetic resonance imaging (MRI) diagnosis in HCC. GE11-CSO-SPION micelles were prepared and characterized. MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was used to analyze micellar cytotoxicity. Fluorescence analysis of cellular uptake was used to analyze the targeting of GE11 peptides to liver cancer cells. Prussian blue staining was used to observe the binding of cells and micelles, and the imaging ability was tested by MRI in vitro and in vivo. GE11-CSO-SPION micelles were spherical, with uniform size and no obvious aggregation of nanoparticles. No obvious micellar cytotoxicity was observed. Cellular uptake showed that GE11 peptide was specific to SMMC-7721 cells and HepG2 cells with high EGFR expression. Prussian blue staining showed that the uptake of SPION micelles by HepG2 cells was good. In vitro and in vivo MRI showed that GE11-CSO-SPION micelles had good imaging effects. GE11-CSO-SPION micelles can be used as an MRI contrast agent to detect HCC with high EGFR expression, providing reliable data for early diagnosis of HCC.

【 授权许可】

CC BY