Genetic diversity and drug susceptibility of Mycobacterium tuberculosis in a city with a high prevalence of drug resistant tuberculosis from Southeast of Mexico

BMC Infectious Diseases

Daniela Munro-Rojas¹  Ana C. Jimenez-Ruano²  Hilda Montero³  Damián Pérez-Martínez⁴  Esdras Fernandez-Morales⁵  Roberto Zenteno-Cuevas⁶  Ángel Trigos⁷  Everest de Igartua⁸  Leila Escobar⁸  Javier Fuentes-Dominguez⁸ 

[1] Anáhuac University, Xalapa, Veracruz, Mexico;Master of Health Science Program, Health Sciences Institute, University of Veracruz, Xalapa, Veracruz, Mexico;Public Health Institute, University of Veracruz, Av. Luis Castelazo Ayala S/N, A.P. 57, Col. Industrial Ánimas, Xalapa, 91190, Veracruz, México;Public Health Institute, University of Veracruz, Av. Luis Castelazo Ayala S/N, A.P. 57, Col. Industrial Ánimas, Xalapa, 91190, Veracruz, México;Doctorate in Health Sciences Program, Health Sciences Institute, University of Veracruz, Veracruz, Mexico;Public Health Institute, University of Veracruz, Av. Luis Castelazo Ayala S/N, A.P. 57, Col. Industrial Ánimas, Xalapa, 91190, Veracruz, México;Master of Health Science Program, Health Sciences Institute, University of Veracruz, Xalapa, Veracruz, Mexico;Public Health Institute, University of Veracruz, Av. Luis Castelazo Ayala S/N, A.P. 57, Col. Industrial Ánimas, Xalapa, 91190, Veracruz, México;Multidisciplinary Research Network on Tuberculosis, Veracruz, Mexico;Research Center in Applied Mycology, University of Veracruz, Xalapa, Veracruz, Mexico;Veracruz Health Department, Veracruz, Mexico;

关键词: Spoligotyping;    MIRU-VNTR;    Genotyping;    Tuberculosis;    Mexico;   

DOI  :  10.1186/s12879-021-06904-z

来源: Springer

【 摘 要 】

BackgroundMexico is on the top five countries with the highest number of TB cases in America continent, nevertheless, information about genotypes circulating is practically unknown. Considering the above this study aims to characterize the genetic diversity of TB in the city of Veracruz, México.MethodsA cross-sectional study was conducted among positive smear samples from patients living in Veracruz City, samples were cultured, and first-line drug profiles determined. Genotyping was made by spoligotyping and MIRU-VNTR 24 loci. Associations of lineages, clusters, and variables were also analyzed.ResultsAmong the 202 isolates analyzed resistance to at least one drug was observed in 60 (30%) isolates and 41(20%) were multidrug-resistant. Three major lineages were identified: L4/Euro-American (88%), L1/Indo-Oceanic (9%), and L2/East Asian (3%). The Euro-American lineage included more than six sublineages, the most abundant were: H (32%), T (23%), LAM (18%), and X (12%). 140 isolates (70%) were placed in 42 SITs patterns.ConclusionsThese results provide the first baseline data on the genetic structure of TB in the city of Veracruz. Sublineages H, X and LAM were predominant; however, it was founded an important diversity of genotypes that could contribute to the dispersion of TB and explain the high prevalence. This information might be useful for the development of further interventions to reduce impact of TB.

【 授权许可】

CC BY