Rotavirus genotyping in gastroenteritis cases of an infantile population from Western Brazilian Amazonia

Revista da Sociedade Brasileira de Medicina Tropical

Maria Sandra Moura Costa²  Paulo Afonso Nogueira²  Gleicienne Félix Magalhães²  Paula Taquita¹  Luis André Mariúba¹  Marilene Penatti¹  Patrícia Puccinelli Orlandi² 

[1] ,Centro de Pesquisa em Medicina Tropical Setor de Microbiologia Porto Velho RO

关键词: Group A rotavirus;    Genotyping;    Brazilian Amazonia;    Rotavírus grupo A;    Genotipagem;    Amazônia brasileira;   

DOI  :  10.1590/S0037-86822012000400021

来源: SciELO

【 摘 要 】

INTRODUCTION: During the period from 2000 to 2002, 79 rotavirus-positive stool samples were collected from children presenting diarrhea in the Western Brazilian Amazon. METHODS: Molecular characterization of the G and P genotypes was performed using RT-PCR and electropherotyping analysis by polyacrylamide gel electrophoresis. RESULTS: A total of 59 samples were confirmed as group A rotavirus. A long electrophoretic profile was exhibited by the G1P[8], G3P[8], and G4P[8] genotypes. The G1P[8] genotype was found in greater proportion. The short electropherotype was exhibited only by G2 genotype strains. CONCLUSIONS: The proportion of the rotavirus genotypes observed was not different from that in other areas of Brazil. This study is the first genotyping of rotavirus in the Western Brazilian Amazon.

【 授权许可】

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