Rotavirus genotyping in gastroenteritis cases of an infantile population from Western Brazilian Amazonia

Revista da Sociedade Brasileira de Medicina Tropical

Taquita, Paula¹  Magalhães, Gleicienne Félix¹  Penatti, Marilene¹  Fundação Oswaldo Cruz, Manaus¹  Hospital Infantil Cosme Damião, Porto Velho¹  Centro de Pesquisa em Medicina Tropical, Porto Velho¹  Mariúba, Luis André¹  Orlandi, Patrícia Puccinelli¹  Costa, Maria Sandra Moura¹  Nogueira, Paulo Afonso¹ 

关键词: Group A rotavirus;    Genotyping;    Brazilian Amazonia;   

DOI  :  10.1590/S0037-86822012000400021

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Medicina Tropical

【 摘 要 】

INTRODUCTION: During the period from 2000 to 2002, 79 rotavirus-positive stool samples were collected from children presenting diarrhea in the Western Brazilian Amazon.METHODS: Molecular characterization of the G and P genotypes was performed using RT-PCR and electropherotyping analysis by polyacrylamide gel electrophoresis.RESULTS: A total of 59 samples were confirmed as group A rotavirus. A long electrophoretic profile was exhibited by the G1P[8], G3P[8], and G4P[8] genotypes. The G1P[8] genotype was found in greater proportion. The short electropherotype was exhibited only by G2 genotype strains.CONCLUSIONS: The proportion of the rotavirus genotypes observed was not different from that in other areas of Brazil. This study is the first genotyping of rotavirus in the Western Brazilian Amazon.

【 授权许可】

Unknown