Brazilian Archives of Biology and Technology | |
Semliki forest virus as a vector: pros and cons for its use in biopharmaceuticals production | |
Eutimio Gustavo Fernández Núñez2  Soraia Attie Calil Jorge1  Renato Mancini Astray1  Alexandre Gonçalves De Rezende1  Bruno Labate Vale Da Costa1  Daniella Cristina Ventini Monteiro2  Carlos Augusto Pereira2  Aldo Tonso2  | |
[1] ,Universidade de São Paulo Escola Politécnica Departamento de Engenharia QuímicaSão Paulo SP ,Brasil | |
关键词: BHK-21; Large-scale bioprocesses; Mammalian cells; Recombinant proteins; SFV; Transient expression; | |
DOI : 10.1590/S1516-89132013000500018 | |
来源: SciELO | |
【 摘 要 】
The number of biopharmaceuticals for medical and veterinarian use produced in mammalian cells is increasing year after year. All of them are obtained by stable recombinant cell lines. However, it is recognized that transient gene expression produces high level expression in a short time. In that sense, viral vectors have been extensively used for producing recombinant proteins on lab-scale. Among them, Semliki Forest virus is commonly employed for this purpose. This review discusses the main aspects related to the use of Semliki Forest virus technology as well as its advantages and drawbacks which limit currently its utilization in biopharmaceutical industry on large-scale.
【 授权许可】
CC BY-NC
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
【 预 览 】
Files | Size | Format | View |
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RO202005130166663ZK.pdf | 104KB | download |