Semliki forest virus as a vector: pros and cons for its use in biopharmaceuticals production

Brazilian Archives of Biology and Technology

Eutimio Gustavo Fernández Núñez²  Soraia Attie Calil Jorge¹  Renato Mancini Astray¹  Alexandre Gonçalves De Rezende¹  Bruno Labate Vale Da Costa¹  Daniella Cristina Ventini Monteiro²  Carlos Augusto Pereira²  Aldo Tonso² 

[1] ,Universidade de São Paulo Escola Politécnica Departamento de Engenharia QuímicaSão Paulo SP ,Brasil

关键词: BHK-21;    Large-scale bioprocesses;    Mammalian cells;    Recombinant proteins;    SFV;    Transient expression;   

DOI  :  10.1590/S1516-89132013000500018

来源: SciELO

【 摘 要 】

The number of biopharmaceuticals for medical and veterinarian use produced in mammalian cells is increasing year after year. All of them are obtained by stable recombinant cell lines. However, it is recognized that transient gene expression produces high level expression in a short time. In that sense, viral vectors have been extensively used for producing recombinant proteins on lab-scale. Among them, Semliki Forest virus is commonly employed for this purpose. This review discusses the main aspects related to the use of Semliki Forest virus technology as well as its advantages and drawbacks which limit currently its utilization in biopharmaceutical industry on large-scale.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License