Caveolin-1 is involved in encephalomyocarditis virus replication in BHK-21 cells

Virology Journal

Amjad Ali¹  Kexue Zhao²  Qiongyi Li²  Yang Liu²  Shujuan Xu²  Jialin Bai²  Rongxiu Liu³  Ying Ling³ 

[1] Biomedical Research Center, Key Laboratory of Biotechnology and Bioengineering of State Ethnic Affairs Commission, Northwest Minzu University, Lanzhou, China;Biomedical Research Center, Key Laboratory of Biotechnology and Bioengineering of State Ethnic Affairs Commission, Northwest Minzu University, Lanzhou, China;College of Life Science and Engineering, Northwest Minzu University, Lanzhou, China;College of Life Science and Engineering, Northwest Minzu University, Lanzhou, China;

关键词: Endocytosis;    Encephalomyocarditis virus;    BHK-21;    Caveolin-1;    Clathrin;    Macropinocytosis;    Dynamin;    Actin;   

DOI  :  10.1186/s12985-021-01521-3

来源: Springer

【 摘 要 】

BackgroundEncephalomyocarditis virus, member of Cardiovirus genus within Picornaviridae family, is an important pathogen that infects different domestic and wild animals. However, the molecular mechanism of its entry remains unclear. In this study, we investigated the mechanism of EMCV infectivity in relation to endocytic pathway using BHK-21 cells.MethodsThe function of numerous cellular key factors implicated in the various endocytic mechanisms were systematically explored using chemical inhibitors. Furthermore, RNA interference (RNAi) as well as the overexpression of dominant protein combined to virus infectivity assays, and confocal microscopy was used to examine EMCV infection in details.ResultsThe results indicated that the EMCV entry into BHK-21 cells depends on caveolin, dynamin, and actin but not clathrin nor macropinocytosis pathways. The effects of overexpression and knockdown of caveolin-1, one components of the caveolae, was examined on EMCV infection. The results showed that EMCV infection was positive correlation with caveolin-1 expression. Confocal microscopy analysis and internalization assay showed that caveolin-1 is required at the early stage of EMCV infection.ConclusionsCaveolin-1, dynamin, and actin-dependent endocytosis pathways are necessary for EMCV infection in vitro.

【 授权许可】

CC BY