Production of bioactive trastuzumab and chimeric anti-VEGF antibody in the cytoplasm of Escherichia coli

4th International Conference on Agricultural and Biological Sciences

农业科学;生物科学

Charoenpun, P.^1 ; Leelawattanachai, J.^2 ; Meechai, A.^1,3 ; Waraho-Zhmayev, D.^1

Biological Engineering Program, Faculty of Engineering, King Mongkut's University of Technology Thonburi, Bangkok

10140, Thailand^1

Nano-Molecular Target Discovery Laboratory, National Nanotechnology Center, National Science and Technology Development Agency, Pathum Thani

12120, Thailand^2

Department of Chemical Engineering, Faculty of Engineering, King Mongkut's University of Technology Thonburi, Bangkok

10140, Thailand^3

关键词: Effect of temperature;    Immunoglobulin G;    Mammalian cells;    Medical fields;    Monoclonal antibodies (mAb);    Research fields;    Trastuzumab;    Western-blot analysis;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/185/1/012003/pdf
DOI  :  10.1088/1755-1315/185/1/012003

来源: IOP

【 摘 要 】

Monoclonal antibody (mAb) is a very useful tool in not only medical field but also in research field. However, the current cost of its production is very high as most mAbs are produced in mammalian cell system. Escherichia coli is a good alternative but has many bottlenecks. Recently, it was demonstrated that biological active full-length Immunoglobulin G (IgG) could be produced in the reductive cytoplasm of an engineered E. coli strain called SHuffle. In this study, we investigated the effect of temperature and induction level on the production of full-length humanized anti-Her2 and chimeric anti-VEGF IgGs expressed as cyclonal. The solubility as well as the assembly of the IgG molecules was examined by western blot analysis. It was found that expression at 30 °C with 0.1 mM IPTG induction was the most suitable for trastuzumab. In contrast, the full-length expression of chimeric anti-VEGF required induction with 1 mM IPTG at 30 °C., however, 1 mM IPTG induction resulted in more full-length IgG. In conclusion, the present study indicated that E coli could be successfully used for the full-length expression of IgG by suitably modifying the expression conditions.