Protective antibody response of Balb/c mice to Bali rabies virus isolate propagated in BHK-21 cells

The Journal of Veterinary Medical Science

Ni Luh Putu AGUSTINI¹  I Nyoman Mantik ASTAWA²  I Wayan MASA TENAYA³ 

[1] Biotechnology Laboratory, Animal Disease Investigation Center, Regional IV. Denpasar Bali, Indonesia;Laboratory of Veterinary Virology, Faculty of Veterinary Medicine, Udayana University, Jln. PB Sudirman, Denpasar, Bali, Indonesia;Undergraduate Student at the Faculty of Veterinary Medicine, Udayana University, Jln. PB Sudirman, Denpasar, Bali, Indonesia

关键词: antibody;    Bali rabies virus isolate;    BHK-21;    mice;    protective antibody response;   

DOI  :  10.1292/jvms.17-0385

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

The protective antibody response of Balb/c mice to Bali rabies virus (RABV) in BHK-21 cells was studied. The virus was isolated from a rabid dog and was adapted to replicate in BHK-21 cell culture for seven passages. The BHK-21-adapted Bali RABV (BHK-Bali RABV) was inactivated with binary ethylenimine and 24 mice were immunized twice at 21-days intervals with the inactivated virus and Rabisin® vaccine. Virus replication was detected using indirect immunofluorescence, immunocytochemistry, and western blotting assays. Enzyme-linked immunosorbent assay examination 2 weeks after the first immunization revealed RABV antibody titers that were mostly below the minimum protective level (<0.5 equivalent unit, EU). Antibody titers increased sharply after the second immunization. Antibody titers in serum of mice induced by inactivated BHK-Bali RABV one week after the second immunization were slightly lower (0.8–3.8 EU) than those induced by Rabisin vaccine (0.9–6.3 EU). RABV antibody titers were stable for at least 6 weeks after the second immunization. Both Rabisin vaccine and inactivated BHK-Bali RABV induced neutralizing antibodies with neutralization titers (50% protective dose per ml) of 29.84 for 0.1 ml Rabisin, 211.41 for 0.2 ml Rabisin, 27.41 for 0.1 ml BHK-Bali RABV, and 28.25 for 0.2 ml BHK-Bali RABV. Thus, inactivated BHK-Bali RABV induces a protective immune response in Balb/c mice, but at lower levels compared to induction by Rabisin vaccine.

【 授权许可】

Unknown