Nanoscale spectroscopy and tomography with the HZB X-ray microscope: Applications in materials and life sciences

11th International Conference on X-ray Microscopy

Guttmann, P.^1 ; Rehbein, S.^1 ; Werner, S.^1 ; Henzler, K.^1 ; Tarek, B.^1 ; Schneider, G.^1

Helmholtz-Zentrum Berlin für Materialien und Energie GmbH, Institute for Soft Matter and Functional Materials, Albert-Einstein-Str. 15, 12489 Berlin, Germany^1

关键词: Adherent cells;    Cell organelle;    Electron storage ring;    Field transmission;    Mammalian cells;    Package density;    Quantitative information;    Tomographic imaging;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/463/1/012032/pdf
DOI  :  10.1088/1742-6596/463/1/012032

来源: IOP

【 摘 要 】

The full-field transmission X-ray microscope at the electron storage ring BESSY II allows nanoscale spectroscopy as well as correlative fluorescence and nanoscale tomographic imaging of frozen-hydrated adherent cells. Reconstructions of tilt series permit the visualization of the sub-cellular structures in mammalian cells and plant cells. Quantitative information about package densities of cell organelles in the cytoplasm is possible. We present an overview of recent results in materials and life sciences obtained with the HZB X-ray microscope.