Calculations of the Acceleration of Centrifugal Loading on Adherent Cells

4th International Conference on Mechanics and Mechatronics Research

机械制造;无线电电子学

Chen, Kang^1 ; Song, Yang^1 ; Liu, Qing^1 ; Zhang, Chunqiu^1

Tianjin Key Laboratory of the Design, Intelligent Control of the Advanced Mechatronical System, School of Mechanical Engineering, Tianjin University of Technology, Tianjin

300384, China^1

关键词: Adherent cells;    Cell loading;    Centrifugal loading;    Centripetal acceleration;    Constant acceleration;    High acceleration;    Living cell;    Mechano-biology;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/224/1/012053/pdf
DOI  :  10.1088/1757-899X/224/1/012053

来源: IOP

【 摘 要 】

Studies have shown that the morphology and function of living cells are greatly affected by the state of different high acceleration. Based on the centrifuge, we designed a centrifugal cell loading machine for the mechanical biology of cells under high acceleration loading. For the machine, the feasibility of the experiment was studied by means of constant acceleration or variable acceleration loading in the Petri dish fixture and/or culture flask. Here we analyzed the distribution of the acceleration of the cells with the change of position and size of the culturing device quantitatively. It is obtained that Petri dish fixture and/or culture flask can be used for constant acceleration loading by experiments; the centripetal acceleration of the adherent cells increases with the increase of the distance between the rotor center of the centrifuge and the fixture of the Petri dish and the size of the fixture. It achieves the idea that the general biology laboratory can conduct the study of mechanical biology at high acceleration. It also provides a basis for more accurate study of the law of high acceleration on mechanobiology of cells.