Quantitative study of mammalian cells by scanning transmission soft X-ray microscopy

X-Ray Microscopy Conference 2016

Shinohara, K.^1 ; Ohigashi, T.^2 ; Toné, S.^3,5 ; Kado, M.^4 ; Ito, A.^1

School of Engineering, Tokai Univ, Kanagawa, Hiratsuka

259-1292, Japan^1

UVSOR Synchrotron, Inst Molecular Science, Aichi, Okazaki

444-8585, Japan^2

Dept Biochem, Kawasaki Medical School, Okayama, Kurashiki

701-0192, Japan^3

Kansai Photon Science Institute, QST, Kizugawa, Kyoto

619-0215, Japan^4

Tokyo Denki Univ, Hatoyama, Saitama

350-0394, Japan^5

关键词: Absorption edges;    Bovine serum albumins;    Mammalian cells;    Molecular components;    Molecular distribution;    Quantitative study;    Soft x-ray microscopy;    Transmission microscopy;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/849/1/012003/pdf
DOI  :  10.1088/1742-6596/849/1/012003

来源: IOP

【 摘 要 】

Molecular distribution in mammalian cells was studied by soft X-ray scanning transmission microscopy with respect to the quantitative aspect of analysis. NEXAFS profiles at the C, N and O K-absorption edges were combined and used for the analysis. For the estimation of quantity for nucleic acids and proteins, NEXAFS profiles of DNA and bovine serum albumin (BSA) at the N K-absorption edge were applied assuming that those were their representatives. The method has a potential to explore the other molecular components than nucleic acids and proteins.