L-arginine in the ischemic phase protects against liver ischemia-reperfusion injury

Acta Cirurgica Brasileira

Murched Omar Taha²  Afonso Caricati-neto¹  Regiane Miranda Ferreira¹  Manuel De Jesus Simões¹  Hugo Pequeno Monteiro²  Djalma José Fagundes² 

[1] ,UNIFESP Department of Surgery Operative Technique and Experimental Surgery DivisionSao Paulo SP ,Brazil

关键词: Nitric Oxide;    Arginine;    Warm Ischemia;    Cytokines;    Antioxidants;    Interleukin-6;    Liver;    Rabitts;    Óxido Nítrico;    Arginina;    Isquemia Quente;    Citocinas;    Antioxidantes;    Interleucina-6;    Fígado;    Coelhos;   

DOI  :  10.1590/S0102-86502012000900005

来源: SciELO

【 摘 要 】

PURPOSE: To investigate the effects of intravenous L-arginine (LG) infusion on liver morphology, function and proinflammatory response of cytokines during the early phase of ischemia-reperfusion injury (IRI). METHODS: Thirty rabbits were subjected to 60 minutes of hepatic ischemia and 120 minutes of reperfusion. An intravenous injection of saline or L-arginine was administered five minutes before the ischemia and five minutes before initiating the reperfusion and at the 55th and 115th minutes after the ischemia. Samples were collected for histological analysis of the liver and measurements of the serum AST, ALT and LDH and the cytokines IL-6 and TNF-alpha. RESULTS: It was observed a significant reduction of sinusoidal congestion, cytoplasmic vacuolization, infiltration of polymorphonuclear leukocyte, nuclear pyknosis, necrosis and steatosis in liver tissue, as well as AST, ALT and LDH after injection of LG in the ischemia (p <0.001). Lower levels of IL-6 and TNF-alpha were associated with LG infusion during ischemia. Higher levels these proteins were observed in animals receiving LG during reperfusion. CONCLUSION: L-arginine protects the liver against ischemia/reperfusion injury, mainly when is administered during the ischemic phase.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License