Acta Cirurgica Brasileira | |
L-arginine in the ischemic phase protects against liver ischemia-reperfusion injury | |
Murched Omar Taha2  Afonso Caricati-neto1  Regiane Miranda Ferreira1  Manuel De Jesus Simões1  Hugo Pequeno Monteiro2  Djalma José Fagundes2  | |
[1] ,UNIFESP Department of Surgery Operative Technique and Experimental Surgery DivisionSao Paulo SP ,Brazil | |
关键词: Nitric Oxide; Arginine; Warm Ischemia; Cytokines; Antioxidants; Interleukin-6; Liver; Rabitts; Óxido Nítrico; Arginina; Isquemia Quente; Citocinas; Antioxidantes; Interleucina-6; Fígado; Coelhos; | |
DOI : 10.1590/S0102-86502012000900005 | |
来源: SciELO | |
【 摘 要 】
PURPOSE: To investigate the effects of intravenous L-arginine (LG) infusion on liver morphology, function and proinflammatory response of cytokines during the early phase of ischemia-reperfusion injury (IRI). METHODS: Thirty rabbits were subjected to 60 minutes of hepatic ischemia and 120 minutes of reperfusion. An intravenous injection of saline or L-arginine was administered five minutes before the ischemia and five minutes before initiating the reperfusion and at the 55th and 115th minutes after the ischemia. Samples were collected for histological analysis of the liver and measurements of the serum AST, ALT and LDH and the cytokines IL-6 and TNF-alpha. RESULTS: It was observed a significant reduction of sinusoidal congestion, cytoplasmic vacuolization, infiltration of polymorphonuclear leukocyte, nuclear pyknosis, necrosis and steatosis in liver tissue, as well as AST, ALT and LDH after injection of LG in the ischemia (p <0.001). Lower levels of IL-6 and TNF-alpha were associated with LG infusion during ischemia. Higher levels these proteins were observed in animals receiving LG during reperfusion. CONCLUSION: L-arginine protects the liver against ischemia/reperfusion injury, mainly when is administered during the ischemic phase.
【 授权许可】
CC BY
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