Effect of L-Arginine and L-NAME treatments on polymorphonuclear leukocytes and mononuclear cells influx during tumor growth

Acta Cirurgica Brasileira

Olívia Teixeira Gomes Reis²  Juliana Crepaldi Raini²  Silvana Torossian Coradi¹  Dulce Helena Jardim Constantino¹ 

[1],USC Center on Health Sciences Bauru SP ,Brazil

关键词: Carcinoma;    Ehrlich Tumor;    Arginine;    NG-Nitroarginine Methyl Ester;    Leukocytes;    Mononuclear;    Nitric Oxide;    Neutrophils;    Mice;    Carcinoma de Ehrlich;    Arginina;    NG-Nitroarginina Metil Éster;    Leucócitos Mononucleares;    Óxido Nítrico;    Neutrófilos;    Camundongos;   

DOI  :  10.1590/S0102-86502009000200006

来源: SciELO

【 摘 要 】

PURPOSE: Evaluate polymorphonuclear leukocytes (PMN's) and mononuclear cells (MN's) involvement in the Ehrlich´s solid tumor (ET) growth. METHODS: 90 Swiss mice were inoculated with 10(7) tumor cells (sc), distributed in three groups and treated once a day, via intraperitoneal (ip), with 0.1ml of diluent, L-Arginine (20mg/Kg) or L-NAME (20mg/Kg). After 7, 15 and 30 days of treatment, ten animals of each group were euthanized, the tumor mass was removed, processed and fixed for HE. Later, a morphometric analysis of the total area, parenchyma, necrosis, tumor stroma and PMN's leukocytes and MN's cells influx was performed. RESULTS: The L-Arginine treatment increased PMN's influx in the initial stage, whereas L-NAME reduced it. Our data suggests that NO effect on PMN's migration is dose-dependent. On the other hand, the MN´s cells influx was reduced by L-NAME treatment at all evaluated periods and at the same periods an increase in tumor growth was observed. CONCLUSION: At initial stages of tumor implantation, both PMN's leukocytes and MN's cells act together to control ET development.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License