Identification of Molecular and Cellular Responses of Desulfovibrio vulgaris Biofilms under Culture Conditions Relevant to Field Conditions for Bioreduction of

Wall, Judy D.

University of Missouri--Columbia

关键词: Genes;    59 Basic Biological Sciences;    Nutrients;    Arginine;    Proteins;   

DOI  :  10.2172/896801
RP-ID  :  ERSD-1026866b-2006
RP-ID  :  None
RP-ID  :  896801

美国|英语

来源: UNT Digital Library

【 摘 要 】

Desulfovibrio vulgaris Hildenborough forms biofilms under stress and nutrient limitation conditions. Analysis of the transcriptional responses to acid exposure suggested that genes encoding arginine and polyamine biosynthesis were increased in expression. A literature search showed that polyamines had been suggested to stimulate biofilm formation in some bacteria. Therefore, biofilm formation by D. vulgaris was then examined. Two different assay methods were used to estimate the biofilm formation. First, the classical crystal violet stainable material attached to glass test tubes was measured. Second protein attached to the test tube sides and especially precipitated at the bottom was measured.