Desenvolvimento e validação de método analítico para quantificação do fármaco bevacizumabe por cromatografia a líquido de alta eficiência

Química Nova

Elionai Cassiana De Lima Gomes²  Armando Da Silva Cunha Júnior²  Maria Irene Yoshida¹  Rodrigo Jorge¹ 

[1] ,Universidade Federal de Minas Gerais Faculdade de Farmácia Belo Horizonte MG ,Brasil

关键词: bevacizumab;    high performance liquid chromatography;    validation;   

DOI  :  10.1590/S0100-40422012000300029

来源: SciELO

【 摘 要 】

In this study, an analytical method was developed and validated for quantitation of the drug bevacizumab (Avastin®) by high performance liquid chromatography (HPLC). The HPLC column was a BioSuite 250® HR SEC, 300 x 7.8 mm x 5 µm (Waters, USA). The mobile phase consisted of phosphate buffered saline (PBS). The results revealed that the method was specific, precise, accurate, robust and linear (r² = 0.998) from 5 to 75 µg mL-1. Therefore, this method can be used in drug release studies or in quality control ampoules of the drug.

【 授权许可】

CC BY-NC   
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