Development and validation of an analytical method for quantitation of the drug bevacizumab by high performance liquid chromatography

Química Nova

Yoshida, Maria Irene¹  Universidade de São Paulo, Ribeirão Preto, Brasil¹  Jorge, Rodrigo¹  Universidade Federal de Minas Gerais, Belo Horizonte, Brasil¹  Gomes, Elionai Cassiana de Lima¹  Cunha Júnior, Armando da Silva¹ 

关键词: bevacizumab;    high performance liquid chromatography;    validation.;   

DOI  :  10.1590/S0100-40422012000300029

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

In this study, an analytical method was developed and validated for quantitation of the drug bevacizumab (Avastin®) by high performance liquid chromatography (HPLC). The HPLC column was a BioSuite 250® HR SEC, 300 x 7.8 mm x 5 µm (Waters, USA). The mobile phase consisted of phosphate buffered saline (PBS). The results revealed that the method was specific, precise, accurate, robust and linear (r2 = 0.998) from 5 to 75 µg mL-1. Therefore, this method can be used in drug release studies or in quality control ampoules of the drug.

【 授权许可】

Unknown