A specific polymerase chain reaction method to identify Stenotrophomonas maltophilia

Memórias do Instituto Oswaldo Cruz

Stephanie Wagner Gallo¹  Patricia Locosque Ramos¹  Carlos Alexandre Sanchez Ferreira¹  Silvia Dias De Oliveira¹ 

关键词: Stenotrophomonas maltophilia;    identification;    PCR;   

DOI  :  10.1590/S0074-02762013000300020

来源: SciELO

【 摘 要 】

Stenotrophomonas maltophilia is a multidrug-resistant nosocomial pathogen that is difficult to identify unequivocally using current methods. Accordingly, because the presence of this microorganism in a patient may directly determine the antimicrobial treatment, conventional polymerase chain reaction (PCR) and real-time PCR assays targeting 23S rRNA were developed for the specific identification of S. maltophilia. The PCR protocol showed high specificity when tested against other species of Stenotrophomonas, non-fermentative Gram-negative bacilli and 100 clinical isolates of S. maltophilia previously identified using the Vitek system.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License