Physicochemical properties of chitooligosaccharide prepared by using chitosanase from Stenotrophomonas maltophilia KPU 2123

2nd International Symposium on Marine and Fisheries Research

地球科学;农业科学

Fawzya, Y.N.^1 ; Rahmawati, A.^2 ; Patantis, G.^1

Research Centre for Marine and Fisheries Product Processing and Biotechnology, Jl. K.S. Tubun Petamburan VI, Jakarta, Indonesia^1

Syarif Hidayatullah State Islamic University Jakarta, Jl. Ir. H. Djuanda No. 95, South Tangerang, Banten, Indonesia^2

关键词: Ash contents;    Chitooligosaccharides;    Chitosanase;    Degree of deacetylation;    Enzymatic methods;    Hydrolysis process;    Physicochemical property;    Stenotrophomonas maltophilia;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/139/1/012045/pdf
DOI  :  10.1088/1755-1315/139/1/012045

学科分类：农业科学（综合）

来源: IOP

【 摘 要 】

Study on the physicochemical properties of chitooligosaccharide (COS) prepared by hydrolysis of chitosan using chitosanase from Stenotrophomonas maltophilia KPU 2123 has been carried out. Hydrolysis process was conducted by reacting the soluble chitosan with 8 U•g-1chitosan of chitosanase for 0; 8; 16 and 24 h incubation and stopped by addition of 0.25 M NaOH until reached pH 7. The COS was obtained as supernatant after being centrifugation. The liquid COS were then freeze-dried and analyzed their physicochemical properties, which comprised yield, viscosity, moisture and ash content, the degree of deacetylation (DD), as well as lead (Pb), arsenic (As) content and analyses of COS by Thin Layer Chromatography (TLC). The optimum hydrolysis time was found to be 16 h with the COS viscosity was 8.50 0.87 cPs. The high COS yield was related to high ash content, i.e. 251.70 77.97 % and 50.45 3.19 % (db), respectively. There was lead (Pb) and arsenic (As) metals detected, i.e. 4.4 ppm and 0.1 ppm, respectively. However, they still met the requirement of Pb and As content in a commercial COS referred. Based on the COS properties, desalination process should be applied in the preparation of COS by enzymatic method.