Rapid and Convenient Separation of Chitooligosaccharides by Ion-Exchange Chromatography

5th Annual International Conference on Material Science and Engineering

Wu, Yuxiao^1,2 ; Lu, Wei-Peng^1 ; Wang, Jianing^1 ; Gao, Yunhua^1 ; Guo, Yanchuan^1

Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing

100190, China^1

University of Chinese Academy of Sciences, Beijing

100049, China^2

关键词: Chitobiose;    Chitooligosaccharides;    Chromatographic systems;    Ion exchange chromatography;    Labor intensive;    Large scale productions;    Mass production;    Separation process;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/275/1/012008/pdf
DOI  :  10.1088/1757-899X/275/1/012008

来源: IOP

【 摘 要 】

Pervious methods for separation of highly purified chitooligosaccharides was time-consuming and labor-intensive, which limited the large-scale production. This study developed a convenient ion-exchange chromatography using the ÄKTA™ avant 150 chromatographic system. Five fractions were automatically collected under detecting the absorption at 210 nm. The fractions were analyzed by high-performance liquid chromatography. It proved that they primarily comprised chitobiose, chitotriose, chitotetraose, chitopentaose, and chitohexaose, respectively, with chromatographic purities over 90%. The separation process was rapid, convenient and could be monitored on-line, which would be benefit for the mass production of chitooligosaccharides.