Discrimination of members of the Mycobacterium avium complex by polymerase chain reaction

Revista de Microbiologia

Universidade Federal de São Paulo, São Paulo, Brasil¹  Leão, Sylvia Cardoso¹  Instituto Biológico, São Paulo, Brasil¹  Sircili, Marcelo Palma¹  Roxo, Eliana¹ 

关键词: : amplification;    PCR;    MAC;    Mycobacterium;    identification;   

DOI  :  10.1590/S0001-37141999000200011

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

Mycobacterium avium complex (MAC) species cannot be discriminated by the usual methods of biochemical identification of mycobacteria. This study showed that amplification by PCR of DT1 and DT6, two single copy sequences identified in the genome of M. avium serotype 2, the insertion sequence IS1245, found to be consistently present in M. avium strains and the heat-shock protein gene hsp65, followed by restriction polymorphism analysis, are rapid and accurate tests for the differentiation of the species M. avium, M. intracellulare, and M. scrofulaceum.

【 授权许可】

Unknown