Development of a PCR Test for the Identification of Staphylococcus intermedius Based on the 16S rDNA Sequence

Journal of Veterinary Medical Science

SHIMIZU²  Emi TOMISAKA²  Yoshihisa WAKITA²  Junichi KAWANO²  Václav HÁJEK¹  Eiko MATSUO²  Ryo YASUDA² 

[1] Institute of Microbiology, Faculty of Medicine, Palacky University;Department of Microbiology and Immunology, Faculty of Agriculture, Kobe University

关键词: identification;    PCR;    Staphylococcus intermedius;   

DOI  :  10.1292/jvms.64.603

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(19)Cited-By(5)The development of a PCR assay based on the 16S ribosomal RNA gene (rDNA) sequence was carried out for the identification of Staphylococcus intermedius. Sixty-six strains of S. intermedius, 70 of Staphylococcus aureus and 2 of Staphylococcus hyicus were examined for the assay. The 16S rDNA, of which the PCR target fragment makes up 901 bp corresponding to the sequence data of the gene, was detected in all strains of S. intermedius, but it was not detected in any strains of either S. aureus or S. hyicus. These results suggest that the PCR allows a simple and precise identification of S. intermedius.

【 授权许可】

Unknown