Genome microarray analysis of transcriptional activation in multidrug resistance yeast mutants

FEBS Letters

Goffeau, André³  Balzi, Elisabetta³  DeRisi, Joseph²  Jacq, Claude¹  Marc, Philippe¹  Brown, Patrick²  van den Hazel, Bart³ 

[1] Laboratoire de Génétique Moléculaire, URA 8541 du CNRS, Ecole Normale Supérieure, 46 rue d'Ulm, F-75230 Paris Cedex 05, France;Howard Hughes Medical Institute and Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305-5428, USA;Unité de Biochimie Physiologique, Université Catholique de Louvain, Place Croix du Sud 2/20, B-1348 Louvain-la-Neuve, Belgium

关键词: Yeast;    Multidrug resistance;    Pleiotropic drug resistance;    DNA microarray;   

DOI  :  10.1016/S0014-5793(00)01294-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cDNA from activated mutants of the homologous transcription factors Pdr1p and Pdr3p was used to screen DNA microarrays of the Saccharomyces cerevisiae complete genome. Twenty-six overexpressed targets of the PDR1–3 and/or PDR3–7 mutants were identified. Twenty-one are new targets, the majority of which are of unknown function. In addition to well known ABC transporters, these targets appear to be involved in transport or in membrane lipids and cell wall biosyntheses. Several of the targets seem to contribute to the cell defence against a variety of stresses. Pdr1p and Pdr3p do not act similarly on all targets. Unexpectedly, the expression of 23 other genes appeared to be repressed in the PDR1–3 and/or PDR3–7 mutants. In contrast to the majority of the activated genes, none of the repressed genes contains pleiotropic drug resistance binding sites in their promoter.

【 授权许可】

Unknown