酵母に対するn-アルキル硫酸毒�?�の遺伝子機能情報に基づいたゲノミクス解析

Chem-Bio Informatics Journal

シリサッ�? ソフォン¹  岩橋 均¹  北河 恵美子¹  米�?? 政実² 

[1] 産業�?術総合研究所健康工学研究センター;茨城大学農学部

关键词: Functional genomics;    ファンクショナルゲノミクス;    DNA microarray;    DNAマイクロアレイ;    Toxicity;    毒�?�評価;    n-alkyl sulfates;    陰イオン界面活�?�剤;    Yeast;    酵母;   

DOI  :  10.1273/cbij.8.69

学科分类：生物化学/生物物理

来源: Chem-Bio Informatics Society

【 摘 要 】

References(33)The n-alkyl sulfates (AS) are a class of anionic surfactants that are widely used in industry and in consumer products. In this study, the effects of AS on yeast growth and genome wide transcriptional profiles were analysed by DNA microarray technology. Induced genes were categorized by localization of gene products and by function according to accepted gene ontologies using the MIPS database. A number of genes whose products localized to the cell wall and peroxisome were significantly induced. Genes involved in energy metabolism (i.e., fatty acid &bete;-oxidation pathway) were also significantly induced. To confirm the role of these functions, the sensitivity of selected single gene deletion strains to sodium dodecyl sulfate (SDS) was tested. Deletion strains of cell wall maintenance genes (ΔGAS1,ΔKRE6, and ΔCHS5) were found to be highly sensitive. Interestingly, mutants deleted for genes in the fatty acid &bete;-oxidation pathway were not found to be sensitive. However, regulating genes in the fatty acid &bete;-oxidation pathway were found to respond to SDS exposure in a dose-dependent manner and to be involved in H2O2 production. Here, we report a functional genomics analysis of genome-wide expression data to screen and evaluate AS toxicity in yeast. While the approach begins with a determination of highly-induced genes, its power lies in then determining the most relevant functions targeted by AS, and then assessing loss of key genes by evaluating AS sensitivity in the corresponding deletion mutants.

【 授权许可】

Unknown