Global gene expression during short‐term ethanol stress in Saccharomyces cerevisiae

FEBS Letters

Dequin, S¹  Blondin, B¹  Alexandre, H¹  Ansanay-Galeote, V¹ 

[1] UMR Sciences pour l’œnologie, Microbiologie et Technologie des fermentations, IPV, INRA, Place Viala, 34060 Montpellier Cedex 01, France

关键词: DNA microarray;    Gene expression;    Ethanol stress;    Yeast;   

DOI  :  10.1016/S0014-5793(01)02503-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

DNA microarrays were used to investigate the expression profile of yeast genes in response to ethanol. Up to 3.1% of the genes encoded in the yeast genome were up-regulated by at least a factor of three after 30 min ethanol stress (7% v/v). Concomitantly, 3.2% of the genes were down-regulated by a factor of three. Of the genes up-regulated in response to ethanol 49.4% belong to the environmental stress response and 14.2% belong to the stress gene family. Our data show that in addition to the previously identified ethanol-induced genes, a very large number of genes involved in ionic homeostasis, heat protection, trehalose synthesis and antioxidant defence also respond to ethanol stress. It appears that a large number of the up-regulated genes are involved in energy metabolism. Thus, ‘management’ of the energy pool (especially ATP) seems to constitute an ethanol stress response and to involve different mechanisms.

【 授权许可】

Unknown